Oligomer 6.0

Oligomer is a modern tool for designing and evaluating oligonucleotides—primers, probes, and adapters—used in PCR, qPCR, sequencing, and diagnostic assays. Developed by Ruslan Kalendar, it combines fast, reliable algorithms with an intuitive interface to help researchers move from concept to validated oligos with confidence. Oligomer streamlines routine tasks and supports advanced optimization for complex targets and multiplex panels.

Key capabilities:

• Primer and probe design with flexible constraints for length, GC content, and melting temperature
• Accurate Tm calculations with concentration and salt corrections
• Secondary structure analysis, including hairpins and homo-/heterodimer checks
• Specificity assessment against reference sequences to reduce off-target effects
• Multiplex design with cross-dimer screening to improve panel performance
• Batch processing for high-throughput projects and panel development
• Clear visualizations of binding sites and predicted amplicons
• Exportable reports and sequence outputs for documentation and collaboration

Whether you are building a new assay or scaling a high-throughput pipeline, Oligomer helps you design robust oligos faster and with fewer iterations.

Oligomer is developed by Ruslan Kalendar. The most popular version of this product among our users is 6.0.